Figure 6.
Praziquantel (PZQ) suppresses the activation of other fibroblast‐like cell or fibroblast by inhibiting the TGF‐β/Smad signalling pathway. (a, d) Quantitative real‐time PCR analysis of collagen type I α1 (COL1A1), α‐smooth muscle actin (α‐SMA), and Smad7 mRNA expression in MES13 and NIH3T3 cells. The ΔΔCt method was used to quantify relative changes (n = 6). (b, e) Western blot for COL1A1, α‐SMA, Smad7, and a loading control (GAPDH) protein levels in MES13 and NIH3T3 cells. (c, f) Quantitative analysis of COL1A1, α‐SMA, Smad7, and GAPDH expression (n = 5). All data are presented as means ± SEM. *P < .05, significantly different as indicated; ns, non‐significant