Figure 5.
Resveratrol (Res) inhibited cell proliferation, suppressed epithelial–mesenchymal transition, and prevented extracellular matrix accumulation in TGF‐β1‐treated tubular epithelial cells. (a) TGF‐β1‐induced proliferation of NRK‐52E cells was inhibited by Res treatment. (b) The TGF‐β1‐mediated proliferation of NRK‐52E cells was inhibited by Res. Bar = 20 μm. (c) Res down‐regulated the expression of c‐Myc, cyclin D1, and Bcl‐2 in TGF‐β1‐treated NRK‐52E cells. (d) Res inhibited the expression of α‐smooth muscle actin (α‐SMA), vimentin, and type I and III collagen but induced the expression of E‐cadherin in TGF‐β1‐treated NRK‐52E cells. (e) Res reduced the expression of α‐SMA and type III collagen but increased the expression of E‐cadherin in TGF‐β1‐treated NRK‐52E cells. Bar = 20 μm. Res (HD), resveratrol (100 μmol·L−1); Res (LD), resveratrol (10 μmol·L−1). Data are expressed as means ± SEM in quintuplicate for the cell line experiments. *P < .05, compared with the control group; # P < .05, compared with the TGF‐β1‐treated group