Table 3.

Fixed concentrations, EC₅₀s, and αs for all combinations of Site 1, 2, and 3 anaesthetics

Site 1	Site 2	Site 3	α	Average α
[Etomidate] μM	[R‐mTFD‐MPAB] μΜ	[Alphaxalone] μΜ
0.555	—	0.29 ± 0.03	0.45 ± 0.02
1.11	—	0.10 ± 0.02	0.57 ± 0.01
1.665	—	0.03 ± 0.01	0.77 ± 0.00
0.74 ± .08	—	0.355	0.58 ± 0.04
0.26 ± 0.03	—	0.71	0.62 ± 0.01
0.31 ± .04	—	1.065	0.89 ± 0.02	0.65 ± 0.16 a
0.22	1.33 ± 0.20	—	0.67 ± 0.08
0.55	1.16 ± 0.22	—	0.75 ± 0.09
1.2	0.25 ± 0.05	—	0.65 ± 0.02
1.32 ± 0.17	0.25	—	0.70 ± 0.08
0.76 ± 0.10	0.625	—	0.61 ± 0.04
0.23 ± 0.04	1	—	0.53 ± 0.02	0.69 ± 0.09 a
—	0.585	0.79 ± 0.10	0.80 ± 0.07
—	1.17	0.31 ± 0.05	0.72 ± 0.03
—	1.755	0.21 ± 0.03	0.90 ± 0.02
—	1.35 ± 0.15	0.355	0.83 ± 0.06
—	0.31 ± 0.56	0.71	0.63 ± 0.04
—	0.09 ± 0.01	1.065	0.79 ± 0.01	0.78 ± 0.10 a
0.111	0.30 ± 0.03	0.07	0.23 ± 0.01
0.222	0.12 ± 0.02	0.14	0.25 ± 0.10
0.555	0.01 ± 0.01	0.355	0.51 ± 0.01
0.111	0.12	0.26 ± 0.01	0.28 ± 0.02
0.222	0.23	0.34 ± 0.01	0.44 ± 0.03
0.555	0.585	0.03 ± 0.01	0.52 ± 0.01
0.43 ± 0.06	0.12	0.07	0.29 ± 0.03
0.96 ± 0.10	0.23	0.14	0.63 ± 0.04
0.29 ± 0.04	0.585	0.355	0.63 ± 0.02	0.42 ± 0.16 a

Note. α values are reported as ± SD. SD of average αs are calculated according to Equation (2). Each concentration–response curve had 40 animals.

^aDiffers significantly (P < .01) from the value of one expected for additivity.