Table 3.
In vitro models of the BBB, 2D condition.
| Cell populations | 2D models | Disease | Junction proteins |
~TEER (Ω·cm2)
Pe10−6 (cm·s−1) |
References |
|---|---|---|---|---|---|
| hPSC + rat astrocytes | co-culture | AD, MS and brain tumors | occludins, claudins 5, ZO-I |
TEER 700 Pe 0.6 (with sucrose) |
Lippmann et al., 2012 |
| hPSC + pericyte-primed human NPC | co-culture | occludins, claudins 5, ZO-I |
TEER 5350 Pe 0.6 (with sucrose) |
Lippmann et al., 2014 | |
| Cord blood-derived endothelial progenitor cells + pericytes | co-culture | AD, MS | occludins, claudins 5, ZO-I |
TEER 160 Pe 10–20 (with Lucifer yellow) |
Cecchelli et al., 1999; Boyer-Di Ponio et al., 2014 |
| hIPS-EC + hIPS-NSC + astrocytes + pericytes | co-culture | AD, PD | occludins, claudins 3, 4 and 5 ZO-I |
TEER 433–2,489 Pe 1.58 (with Lucifer yellow) Pe 1.33 (with sodium fluorescein) |
Appelt-Menzel et al., 2017 |
They are based on human-induced pluripotent stem (hIPS) cells or progenitor cells.
AD, Alzheimer's disease; hIPS-EC, human-induced pluripotent stem cell-derived BBB endothelial cells; hPSC, human pluripotent stem cells; MS, multiple sclerosis; NPC, neural progenitor cells; NSC, neural stem cells; PD, Parkinson's disease; Pe, permeability; TEER, trans-endothelial resistance; ZO-1, zonula occludens-1.