Fig. 7.

Peroxynitrite is implicated in cFLIP amplification by increased intracellular O₂^•-. (A & B) CEM/Neo cells were pretreated with FeTPPS (50 μM for 4 hrs) or JSH-23 (25 μM for 1hr) followed by incubation with DDC (200μΜ), PMA (62.5 ng/ml), ATN (40μΜ) or ONOO⁻(20 μM). Whole cell lysates were then subjected to Western blot analysis using anti-cFLIP and β-actin expression was used as the loading control.