Fig. 8.

GW downregulates cFLIP and sensitizes primary lymphoma cells to TRAIL mediated cell death. (A) Primary lymphoma cells were exposed to GW or treated with DDC (200μΜ), DPI (5μΜ) and PMA (62.5 ng/ml) for 1 hr and whole cell lysates were analyzed for cFLIP expression by Western blot analysis with β-actin as the loading control. (B) Primary lymphoma cells (2 × 10⁶) were treated with TRAIL (25 ng/ml) for 18 hrs in culture medium or GW medium and cell viability was determined by the MTT assay. Data shown are Mean ± S.D. from primary cells derived from 5 different patients and P value (*** <0.005) was calculated by Ordinary one-way ANOVA using GraphPad Prism.