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. 2017 Nov 1;10(11):11300–11307.

Figure 4.

Figure 4

SIRT6 serves as a direct target of miR-802. A. Bioinformatic analysis using miRDB software (http://mirdb.org/) showed that the 3’-UTR of SIRT6 mRNA carried a putative binding site for miR-802. Mutation of the binding site (underlined) was achieved with the QuikChange site-direct mutagenesis kit. B. Western blot analysis of SIRT6 protein levels in INS-1 cells transfected with control miR or miR-802 mimic. Numbers indicate fold change in protein levels relative to control. C. Luciferase reporter assay. HEK-293T cells were transfected with wild-type or mutant SIRT6 3’-UTR reporters (0.1 μg) together with pRL-TK (0.02 μg) as well as miR-802 mimic or control miR (50 nM). Luciferase activities were measured 48 h posttransfection. *P < 0.05.