Table 1.
Antibodies used in the present study
| Antibody | Cat. NO | Source | Manufacturer | Western dilution | Immunofluorescence dilution | |
|---|---|---|---|---|---|---|
|
| ||||||
| HRMECs | Retina | |||||
| Anti-actin | #4970S | Rabbit* | CST | 1:1000 | ||
| Anti-FAK | #3285S | Rabbit* | CST | 1:1000 | ||
| Anti-p-FAK | #8556S | Rabbit* | CST | 1:1000 | ||
| Anti-Src | #2108S | Rabbit* | CST | 1:1000 | 1:100 | |
| Anti-p-Src | #6943S | Rabbit* | CST | 1:1000 | ||
| Anti-PLCγ1 | #5690S | Rabbit* | CST | 1:1000 | ||
| Anti-p-PLCγ1 | #8713S | Rabbit* | CST | 1:1000 | ||
| Anti-p38 | #8690S | Rabbit* | CST | 1:1000 | ||
| Anti-p-p38 | #4511S | Rabbit* | CST | 1:1000 | ||
| Anti-p-Akt | #4060S | Rabbit* | CST | 1:1000 | ||
| Anti-Akt | #4691S | Rabbit* | CST | 1:1000 | ||
| Anti-VE-Cadherin | ab33168 | Rabbit* | Abcam | 1:1000 | 1:200 | |
| Anti-Occludin | No. 331588 | Mouse* | Invitrogen | 1:1000 | 1:1000 | 1:1000 |
| Anti-ZO-1 | ab150266 | Mouse* | Abcam | 1:1000 | 1:1000 | 1:1000 |
| FITC-conjugated donkey anti-rabbit-tetramethyl rhodamine isothiocyanate | ab6881 | Abcam | 1:5000 | 1:1000 | 1:1000 | |
| TRITC-conjugated donkey anti-rabbit-tetramethyl rhodamine isothiocyanate | ab6799 | Abcam | 1:5000 | 1:1000 | 1:1000 | |
CST: Cell signaling technology (Danvers, MA, USA); Abcam (Cambridge, MA, USA); Invitrogen (Carlsbad, CA, USA); FAK: focal adhesion kinase; Akt: protein kinase B; Erk: extracellular signal-regulated kinase; VE-Cadherin: vascular endothelial (VE)-Cadherin; ZO-1: zonula occludens-1; PLCγ1: phospholipaseCγ1;
*
monoclonal;
#: polyclonal.