Figure 4.

Genetic silencing of Rab14 recapitulates the tumor suppressor functions of miR-451. (A) The knockdown efficiency of Rab14 in U2OS and Saos-2 cells was tested by Western blotting assay. (B, C) The effects of Rab14 knockdown on cell anoikis of U2OS and Saos-2 cells were determined by caspase-3/7 activity (B) and Annexin V/PI assay (C), respectively. (D, E) U2OS and Saos-2 cells were cotransfected with miR-451 and pcDNA-Rab14 or empty vector. At 48 h after transfection, the cells were collected. And cell anoikis was determined by caspase-3/7 activity (D) and Annexin V/PI assay (E), respectively. (F) The numbers of visible lung metastatic nodules on the surface of the lung as revealed by HE staining. *P < 0.05; **P < 0.01.