Figure 1.

Glo1 is frequently up-regulated in human breast cancer cells and tissues. A. Glo1 mRNA levels in breast cancer cell lines was observably higher than mammary epithelial cell (MCF-10A) analyzed by Real-time PCR. B. The tumor tissue to adjacent nontumorous tissue (ANT) ratio of Glo1 expression was quantified by real-time RT-PCR. C. Glo1 protein was detected by Western blot and also showed that Glo1 protein level in breast cancer cell lines was observably higher than that in mammary epithelial cell (MCF-10A). D. Western blot analysis of Glo1 expression in each of the primary IDC tissue (T) and paired ANT (N) from four patients. E. IHC confirmed that the Glo1 protein was significantly elevated in primary IDC tissues (T) compared to that of paired ANT (N) from eight patients. Magnifications: ×100.