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. 2017 Nov 1;10(11):11288–11299.

Figure 3.

Figure 3

CCL4 promoted angiogenesis in a VEGF-A dependent manner. A and B: AN3CA and HEC-1B cells were pretreated with VEGF-A antibody for 30 mins, followed by stimulation with CCL4 (30, 60, 100 μg/ml) for 24 h, and then the culture medium was collected as conditional medium (CM) and applied to EPCs for 24 h. Tube formation in EPCs was quantified by averaging the length of the tubes in three randomly chosen microscope fields (*P < 0.05, ***P < 0.001).