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. 2017 Nov 1;10(11):11288–11299.

Figure 4.

Figure 4

CCL4 by shRNA inhibited tumor growth and induced cellular apoptosis in vitro. A and B: The efficiency of CCL4 shRNA (sh-CCL4) was detected with RTPCR in AN3CA and HEC-1B cells. C and D: Cell viability was determined for 12 h, 24 h, 36 h, and 48 h using an MTT assay with stable sh-CCL4 expression and control EC cells. E and F: The long-term cell proliferation capacity was determined by a colony formation assay with stable sh-CCL4 expression and control EC cells. G and H: Cell apoptosis was detected using an Annexin V assay with stable sh-CCL4 expression and control EC cells (*P < 0.05).