Figure 3.

The immunohistochemistry results of the neuron morphology and arrangement. A: Normal group: the morphology of normal neuron is complete; B-G: Injured groups (3 h, 6 h, 12 h, 24 h, 3 d, 7 d): the cell arrangement is disorder, the cell nucleus cannot be seen and the morphology of neurons are stained heterogeneous even cannot be observed. (Scale bar =2.5 um); H: Quantified neuron collapse (%) from immunohistochemistry results. Growth cone collapse (%) was up regulated after SCI (3 h, 6 h, 12 h, 24 h, 3 d, 7 d). And in 3 d after SCI, the ratio of collapsed neuron reached peak, more than 60%.