Figure 4.

(A) The results of objective fragment synthesis and identification; (B) The results of plasmid identification; (C, D) Blots of NMII proteins, comparing the efficiency of different siRNAs for silencing NMII gene, the silencing efficiency of three siRNA was 63%, 95% and 79%, respectively; (E, F) The NF200 which labeled neuron was observed, and the neuron expression level was highest in Group A (E: Group A; F: Group B; G: Group C; H: Group D) (scale bar =100 um); (I) Quantified mean optical density for NF200 (lower panel). *P<0.05.