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ROS production and NLRP3 inflammasome expression in diabetic (DM) and non-diabetic (SD) rat wound. The protein level of NLRP3, caspase-1, ASC and IL-1β are measured by Western blot (A-C, J) and. Elisa (D), respectively. The mRNA level of NLRP3, ASC, Caspase1 and IL-1β are measured by real-time PCR (E-H). The intracellular ROS production is measured with fluorescence probe DCFH-DA (I). Data is presented as the means ± SD. *P < 0.05 versus control.
