Figure 2.

Knockdown of DKK1 resulted in decreased ALDH1A1 expression by inactivating p38/JNK/c-Jun signal pathway. A: Knockdown of DKK1 resulted in downregulation of ALDH1A1 mRNA levels. B-D: Representative immunoblots and densitometry analysis of ALDH1A1, phosphorylated p38 (p-p38), p-JNK and p-c-Jun. All samples were collected from Eca109 cells after 24 hours of transfection with DKK1-siRNA and control. Statistically significant differences are indicated: *P < 0.05; Student’s t test. The experiment was repeated at least three times.