Table 3.

Real-time fluorescence quantitative PCR primer sequence

Gene	Primer	Sequences (5’-3’)	Product size (bp)	Tm (°C)
StAR	F	TTCCGACTGGAGGTGCTGCTA	109	55
	R	CCTTGATTTCCTTGACATTTGGGT
CYP11A1	F	CGATGACCTATTCCGCTTTGC	131	56
	R	TGTGGAACATCTGGTAGACGGC
CYP17	F	CAATCTCTGGGCACTGCATCAC	119	56
	R	GCAAGTAACTCTGCGTGGGTGTA
HSD3β	F	GAGTGCCAGCCTTCGTCTACA	151	55
	R	ACTACCTTCTCGGCCATCCTTAT
HSD17β	F	TGACCAAGACCGCCGATGA	149	55
	R	GTACCACTGGCATTGTGATG
β-actin	F	ATCTTCATGGTGCTAGGAGC	135	56
	R	TCCCAGCACCCTTCATAGCAT