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Effect of E2F1 on the expression of nc886 in cervical cancer cells. (A) Sequence analysis of nc886 promoter region. SiHa (B) and HeLa (C) cells were transfected with the recombinant plasmids encoding E2F1 followed by determination of nc886 expression. (D) SiHa cells were exposed to caffeine (25 nM and 50 nM) for 48 h and nc886 levels were then detected. *P<0.05, **P<0.01.
