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E2F1 directly interacted with nc886. A. Construction of recombinant plasmids containing full-length promoter or deleted promoter fragments of nc886. B. Luciferase assay was performed to measure the fluorescent activity after co-transfection with E2F1 and full-length or mutant nc886 promoter luciferase constructs. C. The relative enrichment of nc886 promoter sequence in E2F1-immunoprecipitants was determined by real time PCR and compared to IgG controls. *P<0.05.
