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. 2017 Dec 1;10(12):11461–11470.

Figure 5.

Figure 5

Restoration of PDCD6 in cells overexpressing miR-124 reverses the effects of miR-124 on radiosensitivity. A. The survival fraction of cells treated as described. pcDNA3.1-PDCD6 was transfected into cells continuously overexpressing miR-124 using Lipofectamine 2000. Surviving fraction was calculated as indicated. **P<0.01. B. Western blot analysis of γ-H2AX, Bcl-2, cleaved caspase-3 (c-caspase-3) and PDCD6 expression in CNE-1 and CNE-2 cells treated as described. Cells were exposed to a 4 Gy dose of radiation for another 24 h. All of these proteins were detected 24 h after radiation, except γ-H2AX, which was detected 6 h after exposure to radiation.