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. 2017 Dec 1;10(12):11516–11525.

Figure 3.

Figure 3

miR-138-5p targets hTERT in human CRC cells. A. The seed regions of the miR-138-5p target sites in hTERT. B. A luciferase reporter plasmid containing wild hTERT 3’UTR or mutant hTERT 3’UTR was transfected into 293T cells alone, cotransfected with mimic NC or miR-138-5p mimic, or with inhibitor NC or miR-138-5p inhibitor and luciferase activity was measured. C. qRT-PCR detection of hTERT mRNA expression in miR-138-5p mimics or mimics NC transfected SW480 and HT29 cells. D. Western blot detection of hTERT protein expression in miR-138-5p mimics or mimics NC transfected SW480 and HT29 cells. Data are showed as mean ± S.D. (n = 3). **P<0.01.