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Sequences of Primers Used for real time PCR [30]
| Gene | Direction | Primer sequence (5’-3’) |
|---|---|---|
| TGF-β1 | Forward | GTGGAAATCAACGGGATCAG |
| Reverse | ACTTCCAACCCAGGTCCTTC | |
| MCP-1 | Forward | ATTGGGATCATCTTGCTGGT |
| Reverse | CCTGCTGTTCACAGTTGCC | |
| IL-1β | Forward | GGTCAAAGGTTTGGAAGCAG |
| Reverse | TGTGAAATGCCACCTTTTGA | |
| IL-6 | Forward | CATTTCCACGATTTCCCAGA |
| Reverse | TCCCTCTGTGATCTGGGAAG | |
| TNF-α | Forward | AGGGTCTGGGCCATAGAACT |
| Reverse | CCACCACGCTCTTCTGTCTAC | |
| β-actin | Forward | GGCTGTATTCCCCTCCATCG |
| Reverse | CCAGTTGGTAACAATGCCATGT |
