Table 2.
Summary of observed coefficient of variations (CVs)
| LFQ intra‐ and inter‐day variability | Housekeeping genes | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| VCP | GPI | SNRPD3 | RAB7A | PSMB4 | PSMB2 | VPS29 | REEP5 | CHMP2A | MAP7D1 | ATAD3B | |
| Within day 1 | 2.77 | 2.95 | 13.58 | 5.85 | 10.63 | 9.12 | 12.99 | 10.07 | 11.95 | 17.86 | 23.01 |
| Within day 13 | 3.07 | 3.04 | 5.14 | 4.79 | 4.40 | 5.52 | 4.38 | 9.34 | 12.52 | 13.65 | 14.00 |
| Within day 27 | 3.29 | 5.25 | 5.76 | 6.78 | 9.04 | 10.97 | 11.75 | 8.78 | 6.65 | 23.06 | 26.19 |
| Across days | 3.28 | 3.90 | 11.06 | 7.67 | 8.35 | 9.59 | 10.37 | 10.33 | 11.55 | 20.45 | 20.95 |
| w/o MS variability | 3.58 | 4.48 | 8.83 | 5.95 | 6.22 | 8.25 | 11.05 | 10.17 | 11.59 | 23.59 | 19.18 |
| Overall automated | 3.41 | 4.71 | 9.94 | 6.85 | 9.43 | 9.02 | 10.71 | 11.32 | 11.51 | 21.93 | 25.80 |
| Manual SP3 | 7.14 | 3.88 | 5.16 | 10.45 | 11.35 | 10.01 | 10.37 | 14.12 | 14.29 | 29.74 | 28.85 |
Corresponding to Fig 3B, the table summarizes average coefficient of variation (CV) values of individual selected proteins for individual days, across days, with and without the MS imposed variability, and manual SP3.