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. 2019 Dec 19;19(2):1379–1387. doi: 10.3892/etm.2019.8352

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Copyright: © Li et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 1. — IL-22 expression in osteosarcoma tissues and cell lines. Western blotting and reverse transcription-quantitative PCR were used to detect the expression of IL-22 in osteosarcoma tissues and cell lines. (A) The protein expression of IL-22 in 2 osteosarcoma tissues and adjacent normal tissues was measured using western blotting, and the IL-22 protein/β-actin ratio was calculated. (B) The mRNA expression of IL-22 in 30 osteosarcoma tissues and adjacent normal tissues. (C) The protein expression of IL-22 in normal osteoblast hFOB1.19 cells and osteosarcoma cell lines was measured using western blotting, and the IL-22 protein/β-actin ratio was calculated. (D) The mRNA expression of IL-22 in normal osteoblast hFOB1.19 cells and osteosarcoma cell lines. Experiments were repeated three times. Data are reported as the mean ± standard deviation. **P<0.01 vs. normal tissues. ^##P<0.01 vs. hFOB1.19 cells. IL, interleukin.