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. 2019 Dec 4;19(2):965–971. doi: 10.3892/etm.2019.8282

Figure 4.

Figure 4.

Effect of lncRNA-ATB downregulation on the expression of epithelial to mesenchymal transition-related proteins and the STAT3 pathway activity in SKOV3 cells. (A) LncRNA-ATB-NC or lncRNA-ATB-shRNA was transfected into SKOV3 cells for 48 h, then western blotting was used to detect the protein level of p-STAT3, STAT3, E-cadherin and vimentin in SKOV3 cells. (B) Western blotting results were quantified. RT-qPCR was used to detect the mRNA expression of (C) STAT3, (D) E-cadherin and (E) vimentin in SKOV3 cells. Data are presented as the mean ± SD. Each experiment was repeated three times. *P<0.05 and **P<0.01 vs. control group. E-cadherin, epithelial cadherin; lncRNA-ATB, long non-coding RNA-activated by transforming growth factor-β; lncRNA-ATB-NC, long non-coding RNA-activated by transforming growth factor-β-negative control; SKOV3, ovarian cancer cell line; STAT-3, signal transducer and activator of transcription 3; p-STAT-3, phosphorylated signal transducer and activator of transcription 3.