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. 2019 Dec 18;19(2):1267–1274. doi: 10.3892/etm.2019.8344

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Copyright: © Zhang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 3. — Confocal laser scanning microscopy (CLSM) images displaying the S. aureus biofilm. SYTO9 and PI interacted with the nucleic acids of live bacteria and dead bacteria, which are shown as green and red, respectively. (a, c, e and g) The biofilm was observed under 10× objective; (b, d, f and h) The biofilm was observed under 60× objective. (a and b) The biofilm was treated with PBS. (c and d) The biofilm was treated with 45 mg/ml immunoliposomes. (e and f) The biofilm was treated with 45 mg/ml liposomes. (g and h) The biofilm was treated with 45 mg/ml ISMN. S. aureus, Staphylococcus aureus; ISMN, isosorbide mononitrate.