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. 2019 Dec 5;19(2):999–1005. doi: 10.3892/etm.2019.8294

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Copyright: © Wang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 3. — miR-3182 is a direct target of LINC00858. (A) LINC00858-WT sequence and -Mut sequence containing the miR-3182 binding sites. (B) SO-RB50 and Y79 cells of the miR-3182-transfected group exhibited higher relative miR-3182 expression than those of the miR-NC group. (C) Luciferase reporter assay showed that miR-3182 co-transfection inhibited the luciferase activity of the wt-LINC00858 reporter. (D) SO-RB50 and Y79 cells of the si-LINC00858 group had a significantly higher relative miR-3182 expression when compared with that of the si-NC group. (E) Lower relative LINC00858 expression was observed in SO-RB50 and Y79 cells of the miR-3182-transfected group when compared with that in the miR-NC group. *P<0.05. LINC00858, long intergenic non-protein coding RNA 858; si-LINC00858, small interfering RNA targeting LINC00858; si-NC, scrambled control; miR, microRNA; miR-NC, miR mimics control; WT, wild-type; Mut, mutated.