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. 2020 Jan 10;9:437. doi: 10.3389/fcimb.2019.00437

Figure 3.

Figure 3

The fungal adhesin Als3 mediates adherence to bladder epithelium. (A) GBS recovery from HTB-9 bladder cells when infected alone or with wild-type (DAY185), als3Δ/Δ mutant, or ALS3-complemented (Comp) C. albicans. (B) Wild-type (DAY185), als3Δ/Δ mutant or ALS3-complemented (comp) C. albicans adherence to HTB-9 cells when infected alone or with GBS. Data represent the means from four independent experiments performed in technical duplicate and are expressed as medians with interquartile ranges. (C–G) HTB-9 cells were infected with GFP-expressing GBS only (green) or with GBS and CellTracker Blue-labeled C. albicans strains (blue). (C) Representative binding of GBS and C. albicans to HTB-9 cells. Merged images of green and blue fluorescence are shown in the top panel and merged fluorescence and phase images are shown in the bottom panel, scale bar = 10 μm. Data represents the quantification of (D) average GBS, (E) C. albicans, and (F) Candida-associated GBS in 15 fields/sample. (G) Percentage of total GBS that associates with indicated C. albicans strain. Data represents the combined image counts of two experiments performed in technical duplicate and are expressed as the mean with standard deviation. Statistical analysis was performed using the Kruskal-Wallis ANOVA with Dunn's multiple comparison or Mann-Whitney test, *P < 0.05, **P < 0.01, ****P < 0.0001.