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. 2020 Jan 10;9:1466. doi: 10.3389/fonc.2019.01466

Figure 5.

Figure 5

Interfering B7-H3 expression in CAFs-blocked cytokine secretion and decreased migration and invasion ability. (A) immunofluorescence images of vimentin, CD31, B7-H3, FAP-α, and α-SMA in CAFs and NFs. DAPI fluorescence is shown as blue. Vimentin, CD31, B7-H3, FAP-α, and α-SMA immunofluorescence is shown as red. (B) The Fluorescence intensity statistical results of vimentin, CD31, B7-H3, FAP-α, and α-SMA. Mean ± SEM, *P < 0.05 and #P < 0.01 by Student's t-test. (C) B7-H3 protein expression in fibroblasts after treatment with B7-H3 siRNA. (D) The relative mRNA expression levels of B7-H3 and cytokines are indicated after treatment with B7-H3 siRNA. Mean ± SEM, *P < 0.05 and #P < 0.01 by Student's t-test. (E) The IL-6 and VEGF expression levels as detected by ELISA. Mean ± SEM, *P < 0.05 and #P < 0.01 by Student's t-test. (F) The transwell tests were conducted to assess the migration and invasion ability of fibroblasts.