Figure 1.

Clonogenic survival after pretreatment with volasertib (0–10 nM, 24 h), followed by irradiation (0–8 Gy) in A549, NCI-H1975, A549-NTC, and A549-920 cells: (A) Radiation dose-response curves after the combination treatment. Survival was determined by the clonogenic assay 10 days (d) after irradiation and corrected for the cytotoxic effect of volasertib monotherapy. Data points represent mean values from at least three experiments and are presented as mean ± standard deviation (SD); (B) Representative images of A549 cells after staining with crystal violet 10 d post-irradiation.