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Apoptotic cell death 72 h after pretreatment with volasertib (0–20 nM, 24 h), followed by irradiation (0–6 Gy) in A549 (A), NCI-H1975 (B), A549-NTC (C), and A549-920 (D) cells. Cells were labeled with annexin V-FITC and PI and measured by flow cytometric analysis. Data is presented as the mean percentages of apoptotic cells (i.e., annexin V+/PI− and annexin V+/PI+) after the combination treatment; n > 3.
