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. 2019 Nov 28;11(12):1893. doi: 10.3390/cancers11121893

Table 2.

Percentage of A549, A549-NTC, A549-920, and NCI-H1975 cells in each phase of the cell cycle after pretreatment with volasertib (0–20 nM), followed by radiotherapy (0–6 Gy). PI staining was performed 24 h after irradiation. Data are represented as mean ± SD of at least three experiments.

Cell Percentages in Each Phase of the Cell Cycle
A549 Condition Sub-G1 G0/G1 S G2/M NCI-H1975 Sub-G1 G0/G1 S G2/M
0 nM volasertib → 0 Gy 0.09 ± 0.09 67.88 ± 1.71 14.78 ± 1.40 17.48 ± 0.48 1.42 ± 1.10 58.73 ± 4.46 14.30 ± 1.99 25.70 ± 5.98
7.5 nM volasertib → 0 Gy 0.82 ± 1.21 64.90 ± 2.71 13.23 ± 1.75 20.73 ± 0.74 1.14 ± 0.62 57.83 ± 4.26 13.45 ± 2.11 27.48 ± 5.58
12.5 nM volasertib → 0 Gy 2.65 ± 3.64 61.20 ± 3.35 12.73 ± 1.41 23.18 ± 1.50 2.02 ± 1.37 53.85 ± 3.16 12.64 ± 4.80 31.48 ± 5.62
20 nM volasertib → 0 Gy 3.96 ± 1.02 49.13 ± 3.37 10.95 ± 1.54 35.10 ± 5.94 2.06 ± 1.25 50.18 ± 5.06 12.93 ± 2.15 34.63 ± 2.07
0 nM volasertib → 2 Gy 1.12 ± 1.45 73.40 ± 0.22 8.95 ± 1.45 16.73 ± 2.34 1.61 ± 1.15 58.55 ± 8.01 12.87 ± 2.15 27.00 ± 8.37
7.5 nM volasertib → 2 Gy 0.58 ± 0.37 64.93 ± 4.25 8.76 ± 1.61 25.88 ± 5.12 1.98 ± 1.50 54.70 ± 6.07 10.43 ± 1.66 33.03 ± 5.86
12.5 nM volasertib → 2 Gy 2.10 ± 2.32 59.63 ± 8.45 8.03 ± 1.58 29.88 ± 7.97 2.12 ± 1.88 48.80 ± 2.69 10.32 ± 1.54 38.53 ± 5.33
20 nM volasertib → 2 Gy 2.53 ± 0.88 44.00 ± 6.08 7.65 ± 1.33 44.97 ± 6.80 3.23 ± 1.61 35.33 ± 3.07 11.62 ± 3.15 50.50 ± 8.51
0 nM volasertib → 4 Gy 0.39 ± 0.47 67.33 ± 3.39 5.33 ± 0.74 27.05 ± 4.10 2.37 ± 2.44 49.95 ± 7.47 8.24 ± 2.14 39.33 ± 5.70
7.5 nM volasertib → 4 Gy 1.45 ± 0.95 53.78 ± 8.69 5.31 ± 0.73 39.58 ± 8.43 2.11 ± 0.89 39.23 ± 5.69 7.05 ± 2.83 51.15 ± 5.68
12.5 nM volasertib → 4 Gy 1.22 ± 1.44 49.55 ± 9.11 5.06 ± 1.02 43.98 ± 8.01 2.27 ± 1.86 34.55 ± 5.12 7.67 ± 2.80 55.68 ± 5.98
20 nM volasertib → 4 Gy 3.15 ± 0.26 36.77 ± 7.93 6.84 ± 2.43 53.03 ± 8.89 3.44 ± 1.15 25.00 ± 7.86 9.43 ± 2.95 62.48 ± 8.40
0 nM volasertib → 6 Gy 0.29 ± 0.21 55.88 ± 5.54 3.94 ± 0.42 39.80 ± 5.53 1.83 ± 0.86 34.30 ± 6.00 5.76 ± 1.40 58.30 ± 5.26
7.5 nM volasertib → 6 Gy 0.68 ± 0.69 45.13 ± 8.70 3.32 ± 0.23 50.83 ± 8.25 2.04 ± 0.96 25.15 ± 4.72 6.38 ± 2.56 66.20 ± 5.66
12.5 nM volasertib → 6 Gy 1.08 ± 1.05 41.90 ± 7.49 4.44 ± 0.79 52.55 ± 6.91 1.52 ± 1.26 17.57 ± 2.02 5.34 ± 1.79 75.50 ± 5.11
20 nM volasertib → 6 Gy 3.56 ± 1.02 33.40 ± 7.44 5.82 ± 1.51 57.93 ± 6.83 2.01 ± 0.88 18.95 ± 10.97 6.86 ± 2.87 72.10 ± 12.08
A549-NTC Condition Sub-G1 G0/G1 S G2/M A549-920 Sub-G1 G0/G1 S G2/M
0 nM volasertib → 0 Gy 0.27 ± 0.31 66.30 ± 2.26 16.30 ± 3.24 16.90 ± 0.62 0.45 ± 0.16 66.67 ± 0.83 15.83 ± 2.59 16.77 ± 2.55
7.5 nM volasertib → 0 Gy 0.85 ± 0.38 61.03 ± 3.95 14.50 ± 3.80 23.20 ± 1.73 3.06 ± 1.17 57.20 ± 3.66 17.00 ± 3.46 22.57 ± 4.47
12.5 nM volasertib → 0 Gy 1.37 ± 1.02 57.07 ± 3.74 13.57 ± 1.53 28.87 ± 2.71 2.43 ± 0.37 56.13 ± 2.12 17.07 ± 4.37 24.87 ± 6.37
20 nM volasertib → 0 Gy 3.25 ± 1.60 30.97 ± 5.85 8.48 ± 0.73 57.00 ± 5.02 4.15 ± 2.40 35.93 ± 4.47 14.77 ± 3.01 45.37 ± 5.17
0 nM volasertib → 2 Gy 0.24 ± 0.20 74.77 ± 5.05 9.69 ± 4.05 15.70 ± 1.13 0.47 ± 0.33 68.10 ± 0.79 13.37 ± 3.35 18.27 ± 3.04
7.5 nM volasertib → 2 Gy 0.87 ± 0.46 62.97 ± 3.58 8.83 ± 2.66 27.70 ± 3.13 1.81 ± 0.66 53.23 ± 2.90 12.97 ± 4.06 32.30 ± 3.97
12.5 nM volasertib → 2 Gy 1.37 ± 0.79 57.90 ± 3.40 8.16 ± 3.18 33.40 ± 3.11 2.09 ± 1.00 48.90 ± 2.75 11.00 ± 3.80 37.90 ± 7.04
20 nM volasertib → 2 Gy 2.32 ± 2.28 26.90 ± 5.39 6.21 ± 1.37 65.93 ± 4.31 2.31 ± 0.81 26.30 ± 4.71 12.96 ± 4.27 58.27 ± 5.77
0 nM volasertib → 4 Gy 0.72 ± 0.53 69.77 ± 2.14 4.44 ± 1.35 25.33 ± 1.97 0.54 ± 0.22 63.33 ± 2.53 8.76 ± 1.37 27.97 ± 3.71
7.5 nM volasertib → 4 Gy 0.85 ± 0.55 54.83 ± 3.51 4.67 ± 0.57 39.87 ± 3.65 1.31 ± 0.57 43.70 ± 1.51 8.84 ± 2.03 46.10 ± 1.28
12.5 nM volasertib → 4 Gy 0.77 ± 0.44 45.53 ± 8.47 4.46 ± 1.33 49.60 ± 9.61 1.87 ± 0.18 37.67 ± 2.49 10.36 ± 3.07 50.30 ± 2.59
20 nM volasertib → 4 Gy 1.71 ± 1.21 26.80 ± 1.92 4.24 ± 0.40 67.87 ± 3.09 2.05 ± 0.84 21.17 ± 3.88 12.88 ± 4.43 63.97 ± 4.80
0 nM volasertib → 6 Gy 0.40 ± 0.36 56.40 ± 3.16 3.29 ± 0.56 40.30 ± 3.73 0.62 ± 0.04 52.07 ± 3.74 6.59 ± 0.83 41.17 ± 4.63
7.5 nM volasertib → 6 Gy 0.58 ± 0.67 43.17 ± 3.43 2.96 ± 0.27 53.37 ± 3.56 1.01 ± 0.54 33.80 ± 0.96 7.63 ± 1.53 57.83 ± 1.11
12.5 nM volasertib → 6 Gy 0.82 ± 0.68 40.77 ± 3.43 3.30 ± 0.42 55.33 ± 3.75 1.21 ± 0.47 31.10 ± 4.34 7.72 ± 2.88 60.03 ± 6.72
20 nM volasertib → 6 Gy 1.98 ± 0.70 22.57 ± 3.69 4.67 ± 0.73 71.20 ± 3.13 1.91 ± 1.34 16.80 ± 6.34 9.36 ± 4.49 71.60 ± 10.06

SD: standard deviation.