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. 2019 Nov 28;11(12):1899. doi: 10.3390/cancers11121899

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Histopathological changes in various genetic background zebrafish fed with different diets. Representative H&E stain images and histopathologic change statistics of (A–D) WT, (E–H) HBx(p53-), (I–L) Src(p53-), and (M–P) HBx, Src(p53-) fish after 8 weeks of normal diet (NOR), overfeeding (diet-induced obesity, DIO), or high-fat diet (FAT) starting at 3 months of age, and scarified at 5 months. The histopathologic change consists of percentages in five states: normal, steatosis, hyperplasia, dysplasia, and hepatocellular carcinoma (HCC). The number of fish is represented as N on top of each bar. The scale bar is 50 μm.