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. 2019 Nov 26;11(12):1877. doi: 10.3390/cancers11121877

Figure 1.

Figure 1

Fluorescence microscopy of γ-H2AX and DNA damage response proteins 53BP1, MRE11 and p-ATM in a single alpha-trajectory. (A) Wide-field overview images of human leukocyte nuclei stained with DAPI (blue), γ-H2AX and different proteins (53BP1, MRE11, p-ATM) showing typical regions of interests used for SMLM image acquisition. (B) Representative SMLM signal point density image of a leukocyte nucleus showing several large γ-H2AX super-foci linearly aligned along the trajectory of a single alpha particle. Slice thickness 500 nm. (C) Magnification and signal coordinate representation of red box in (B) showing that one γ-H2AX damage super-focus consists of substructures and smaller regions of high signal densities at the nanoscale.