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. 2019 Dec 4;11(12):1934. doi: 10.3390/cancers11121934

Figure 2.

Figure 2

JAK2 is required for STAT3 phosphorylation and proliferation in HPV+ cervical cancer cells. (A) Growth curve analysis of HeLa (left) and CaSKi (right) cells after addition of inhibitors for 48 h. (B) Growth curve analysis of HeLa (left) and CaSKi (right) after transfection of a pool of four specific JAK2 siRNA for 72 h. (C) Representative western blot of ruxolitinib dose response in HeLa and CaSKi cells after 48 h. Densitometry analysis is in Supplementary Figure S3A. (D) Representative western blot of HeLa and CaSKi cells after transfection of a pool of four specific JAK2 siRNA for 72 h. Densitometry analysis is in Supplementary Figure S3B. (E) Colony formation assay (anchorage dependent growth) of HeLa and CaSKi cells after addition of inhibitors for 48 h. (F) Colony formation assay (anchorage dependent growth) of HeLa and CaSKi cells after transfection of a pool of four specific JAK2 siRNA for 72 h. (G) Soft agar assay (anchorage independent growth) of HeLa and CaSKi cells after addition of inhibitors for 48 h. (H) Soft agar assay (anchorage independent growth) of HeLa and CaSKi cells after transfection of a pool of four specific JAK2 siRNA for 72 h. Error bars represent the mean ± standard deviation of a minimum of three biological repeats. ** p < 0.01, *** p < 0.001 (Student’s t-test).