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. 2019 Dec 6;11(12):1964. doi: 10.3390/cancers11121964

Figure 2.

Figure 2

HCPT inhibits esophageal squamous cell carcinoma cells’ proliferation. (A) Cells’ proliferation of KYSE410, KYSE510, KYSE30, and KYSE450 post HCPT (0, 40, 80, 160, and 320 nM) treatment were detected by MTT assay. Data were shown compared with the dimethyl Sulfoxide (DMSO) treated group. *, p < 0.05; **, p < 0.01; ***, p < 0.001 compared to the controls. (B) Foci formation of ESCC cells were performed in 6-well plates with HCPT (0, 40, 80, and 160 nM) application for 7 days. The colonies number was analyzed and summarized, and the data were shown compared with the DMSO treated group. ***, p < 0.001 compared to controls. (C) Images of crystal violet stained foci after HCPT (0, 40, 80, and 160 nM) treatment for 7 days. (D) Anchorage-independent cell growth assay was performed to evaluate the effect of HCPT (0, 40, 80, and 160 nM) on cell growth. Colonies were captured and the number was counted after 3 weeks; the results are presented as treated group compared with the control group. ***, p < 0.001. (E) Representative pictures of colonies after HCPT treatment on KYSE410, KYSE510, KYSE30, and KYSE450 cells. Three independent repeats were performed for each experiment and were statistically analyzed.