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. 2019 Dec 6;11(12):1964. doi: 10.3390/cancers11121964

Figure 3.

Figure 3

HCPT interrupts ESCC cells’ G2/M cell cycle transition and induces apoptosis. (A) Cell cycle analysis by flow cytometry on ESCC cells after HCPT (0, 80, 160, and 320 nM) treatment for 12 h. Statistics of cell cycle distribution were shown as % cell cycle. **, p < 0.01; ***, p < 0.001 compared to DMSO controls. (B) Western blotting analysis to detect the expression of G2/M phase markers cyclin B1 and p21 after HCPT (0, 80, 160, and 320 nM) treatment in ESCC cells. β-actin was used as an internal reference control. (C) Cell apoptosis were checked by flow cytometry using annexin V and the PI double staining method. The results were summarized and shown as % apoptosis. *, p < 0.05; **, p < 0.01; ***, p < 0.001 compared to the controls. (D) Cells were treated with HCPT (0, 80, 160, and 320 nM) for 72 h. Lysates were harvested and analyzed by Western blotting for the expression of apoptosis markers as indicated. β-actin was used as an internal reference control. Three independent repeats were performed for flow cytometry detection and were statistically analyzed.