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. 2019 Dec 3;11(12):1927. doi: 10.3390/cancers11121927

Figure 3.

Figure 3

Figure 3

TMS-TMF-4f suppresses the STAT3 activation and STAT3-related protein expression in human cervical cancer cells. (a) After treatment with 10 μM TMS-TMF-4f for the indicated times (5, 10, 15, 30, and 60 min), total cellular proteins were prepared, resolved by SDS-PAGE, and detected using specific p-STAT3 and STAT3 antibodies. β-actin was used an internal control. (b) Cells were treated with 10 μM TMS-TMF-4f for 24 h. Total cellular proteins were prepared, resolved by SDS-PAGE, and detected using specific cyclin D1, survivin, and c-Myc antibodies. β-actin was used an internal control. The relative optical density ratio was determined using a densitometric analysis program (Bio-Rad Quantity One® Software, version 4.6.3 (Basic)), normalized to the internal control. After transfection with pMXs-STAT3C (12 μg), the cells were treated with 10 μM TMS-TMF-4f for 24 h. (c) Annexin V-FITC and PI staining and (d) Western blot analyses were performed to detect apoptotic cell death. Data are presented as the mean ± SD of three independent experiments. *** p < 0.001 vs. the pMXs-gw-transfected control group. ### p < 0.001 vs. the TMS-TMF-4f-treated control group.