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. 2019 Nov 22;11(12):1847. doi: 10.3390/cancers11121847

Figure 1.

Figure 1

The ataxia telangiectasia mutated (ATM)–seryl-tRNA synthetase (SerRS)–vascular endothelial growth factor A (VEGFA) pathway played an essential role in UV-induced VEGFA expression in HaCaT cells. (a,b) mRNA levels of VEGFA were analyzed by RT-PCR. (c) VEGFA in supernatants was analyzed via sandwich ELISA. (d) Western blot analysis of P-ATM, ATM, P-SerRS, and SerRS expressions in HaCaT cells with or without UV treatment. (e) Western blot analysis of P-ATM, ATM, P-SerRS, and SerRS expressions in HaCaT Cells with or without treatments of UV and ATMi. (f) VEGFA mRNA levels were detected by RT-PCR. (g) VEGFA in supernatants was analyzed via sandwich ELISA. (h) Western blot analysis of SerRS expression; β-actin served as a loading control. (i) mRNA levels of VEGFA were analyzed by RT-PCR. (j) VEGFA in supernatants was analyzed via sandwich ELISA. All data above are presented as means ± SEM (n = 3, * p < 0.05, ** p < 0.01, *** p < 0.001) of three independent repeats.