Figure 6.

PGE₂ increases the abundance of pro-angiogenic proteins in EOC cells. EOC cells were treated with prostaglandin E2 (PGE₂, 20 μM) for 2 or 24 h. (A–D) β-catenin, c-MYC, and survivin protein levels evaluated by western blotting after PGE₂ stimulation (with the respective protein/β-actin ratios), n = 4 or more (duplicate). (E–H) β-catenin/TCF-Lef transcriptional activity after PGE₂ stimulation (24 h), n = 4. (I–L) VEGF protein levels in culture supernatants of EOC cells stimulated with PGE₂, n = 4 in duplicate. * = p < 0.05 and ** = p < 0.01 with respect to the baseline condition (Kruskal–Wallis test and Dunn´s post-test). Results were expressed as mean ± standard error of the mean (SEM).