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. 2019 Dec 4;11(12):1931. doi: 10.3390/cancers11121931

Figure 9.

Figure 9

Co-culture of H295R cells with mature adipocytes resulted in de-differentiation of the adipose cells. (A) RT-qPCR Taqman assay was performed on cell samples from undifferentiated ASCs, used as negative control, and in vitro differentiated adipocytes cultured alone (mADIPO) or co-cultured with H295R cells (mADIPO+H295R) for 7 days. Gene expression related to AdipoQ and FABP4 is expressed as fold increase (FI) vs. undifferentiated ASCs used as control. (B) Adipocyte lipid content, assessed by AdipoRed assay, was calculated as fold increase (FI) vs. undifferentiated ASCs, (indicated by the white dashed line as = 1). Data are expressed as the mean ± SE in n = 3 independent experiments, ** p < 0.001 vs. mADIPO alone. Representative images of AdipoRed-related fluorescence staining intracellular lipid droplets (green) in samples from undifferentiated ASCs (negative control, (C)), mADIPO (D) and mADIPO+H295R (E). Cell nuclei were counterstained with DAPI (blue). Original magnification: 40×.