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. 2019 Dec 5;11(12):1945. doi: 10.3390/cancers11121945

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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A549 GLUL KO cells displayed sensitivity to glucose deprivation. A549 and H1299 (control and GLUL KO) cells were cultured in various concentrations of glucose as indicated and were treated with 5 mM of 2-DG for 72 h, and the cell viability was analyzed by MTS assay. Data for each pair of cell lines (control/GLUL KO) was normalized to the data for the highest concentration of glucose (25 mM), as shown above. The data are shown as ± standard deviation. The standard deviation (SD) bars in the cell viability graph represent the means of three independent experiments. The data are shown as mean ± SD; p-values were determined using a two-way ANOVA; **** p ≤ 0.001.