Figure 1.

ISO treatment induced tubulin destabilization in MCF7 cells. MCF7 cells were treated with ISO and Rsv for the indicated time points and their roles in tubulin expression was determined using Western blot analysis. (A–C) Protein expression of tubulin and its densitometric analysis. GAPDH was used as a loading control. * p < 0.05, ** p < 0.01, and *** p < 0.001 indicate significant differences compared with untreated control group. (D,E) IF labeling of α and β-tubulin, Scale bars: 50 µm. (F) Tubulin interaction with ISO (pink) and resveratrol (blue). (G) Tubulin docking score.