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. 2019 Dec 5;11(12):1947. doi: 10.3390/cancers11121947

Figure 7.

Figure 7

ISO treatment controlled MAPK/PI3K signaling and downregulated cell proliferation and pro-inflammatory cytokine production. MCF7 cells were treated with ISO and Rsv for 3 h for MAPKs and PI3K, and 48 h for cytokines assay. (A–D) Protein expression of MAPKs and PI3K/AKT and their densitometric analysis. (E) IF labeling of BrdU-positive proliferating cells. (F,G) IF labeling of pERK and pAKT. Scale bars: 50 µM (H–J) Level of secreted pro-inflammatory cytokines, TNF-α, IL-6, and IL-1β. * p < 0.05, ** p < 0.01, and *** p < 0.001 indicate significant differences compared with untreated control group.