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ERK and AKT inhibitor treatment accelerate the anti-cancer effect of ISO in MCF7 cells. MCF7 cells were treated with ISO and EKR inhibitor and AKT inhibitor for 48 h. (A) Cell viability assay. (B) SRB proliferation assay. (C) LDH production assay. * p < 0.05, ** p < 0.01, and *** p < 0.001 indicate significant differences compared with untreated control group. ## p < 0.01 indicates significant differences compared with ISO (40 µM) treated group. (D) Cell morphology.
