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. 2019 Nov 27;11(12):1879. doi: 10.3390/cancers11121879

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Effect of IL-24 on SHH signaling protein in the presence of exogenous Shh treatment. H1299 and A549 cells were treated with or without recombinant Shh (5000 ng/mL), transfected with or without IL-24 plasmid. (A) Western blot was performed to evaluate SMO, GLI1, and PTCH1 protein levels. Beta-actin was used as a protein loading control. (B) Differences in the expression of the proteins were determined by semi-quantitative analysis and represented in graphical format. The experiment was performed at least two times. * Represents p < 0.05, ** represents p < 0.001, *** represents p < 0.0001, ns = not significant. Bars denote standard deviation (SD).