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. 2019 Nov 26;11(12):1871. doi: 10.3390/cancers11121871

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Positive feedback loop between Zta and autophagy initiation. (a) HONE-1 and (b) HA cells were treated with 20 μM C7 for 6, 12, 18, 24, or 30 h. Expression of lysosomal marker LAMP1 (red signals) and autophagosomes marker LC3B (green signals) was analyzed by immunofluorescence staining. DAPI (blue signals) stained cell nuclei. Scale Bar: 10 μm. Percentage of autolysosome formation were counted from 50 cells per time-point. (c) HONE-1 and HA cells were either untreated or treated with 20 μM C7 48 h. Expression of Zta (red signals) and LC3B (green signals) was analyzed by immunofluorescence staining. DAPI (blue signals) stained cell nuclei. Scale Bar: 100 μm. (d) HONE-1 cells transfected with pcDNA3 plasmid express Zta constitutively. Both Zta-transfected HONE-1 and wildtype HA cells were either untreated or treated with 20 μM C7 for 48 h. Expression of Zta (red signals) and LC3B (green signals) was analyzed by immunofluorescence staining. DAPI (blue signals) stained cell nuclei. Scale Bar: 100 μm.

Positive feedback loop between Zta and autophagy initiation. (a) HONE-1 and (b) HA cells were treated with 20 μM C7 for 6, 12, 18, 24, or 30 h. Expression of lysosomal marker LAMP1 (red signals) and autophagosomes marker LC3B (green signals) was analyzed by immunofluorescence staining. DAPI (blue signals) stained cell nuclei. Scale Bar: 10 μm. Percentage of autolysosome formation were counted from 50 cells per time-point. (c) HONE-1 and HA cells were either untreated or treated with 20 μM C7 48 h. Expression of Zta (red signals) and LC3B (green signals) was analyzed by immunofluorescence staining. DAPI (blue signals) stained cell nuclei. Scale Bar: 100 μm. (d) HONE-1 cells transfected with pcDNA3 plasmid express Zta constitutively. Both Zta-transfected HONE-1 and wildtype HA cells were either untreated or treated with 20 μM C7 for 48 h. Expression of Zta (red signals) and LC3B (green signals) was analyzed by immunofluorescence staining. DAPI (blue signals) stained cell nuclei. Scale Bar: 100 μm.