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. 2019 Dec 7;11(12):1967. doi: 10.3390/cancers11121967

Figure 7.

Figure 7

Inhibition of PANX1 channel or genetic ablation of PANX1 gene reduce the invasive and metastatic potential of breast cancer cells. (A) Gelatin zymography of 1 mM PBN-treated MCF-7 cells. Proteins were separated on a gelatin-containing polyacrylamide gel in order to assess the activation status and levels of MMP-2 and MMP-9 enzymes. (B) Bar graph displays densitometry quantification of zymographs of MCF-7 cells, using Image Lab software. Results are representative of three independent experiments. (C,D) are the same as in (A,B), respectively, but for MDA-MB-231 treated with 1 mM PBN and for MDA-PANX1– cells. (E) RTCA measurements of cell invasion of MDA-MB-231 cells treated with 1 mM PBN for 72 h and of MDA-PANX1- cells. Cell impedance readings were taken every 15 m for a minimum of 18 h. Quantification graphs display normalized cell index values, relative to Control. * p < 0.05, ** p < 0.01, and *** p < 0.001.