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. 2019 Dec 5;11(12):1948. doi: 10.3390/cancers11121948

Figure 5.

Figure 5

(a) Cytotoxicity of LUVDOX-TRAIL 1/10 (LDT 1/10) on a panel of tumor cell lines from distinct origin. A panel of tumor cell lines (A549, HT-29, SKBR3, A673, HT-1080, Raji, RD, U937, and MOLT4) were treated with increasing concentrations of LUV-TRAIL (LT), LUVDOX 1/10 (LD 1/10), or LDT 1/10 with entrapped DOX concentration of DOX: 6.45 Μm (DOX 1/10). After 24 h, cell death was measured by annexin-V staining. Graphs show the mean ± SD of at least three independent experiments. * p < 0.05, ** p < 0.01, *** p < 0.001. (b) Cytotoxicity of LDT 1/10 on a panel of tumor cell lines from distinct origin. Raji, U937, and MOLT-4 cells were treated with LT, LD 1/10, or LDT 1/10 for 24 h. Bright field microscopy images of the Control, and cells treated with LD and LDT, were taken. Original magnification was 200×. (c) Comparison of LDT and LDT 1/10 on a panel of tumor cell lines from distinct origin. A panel of tumor cell lines (A549, HT-29, SKBR3, A673, HT-1080, Raji, RD, U937, and MOLT4) were treated with LT, LDT, and LDT 1/10 with different entrapped DOX concentrations (LDT: DOX at 64.56 μM or LDT 1/10: DOX at 6.45 μM). The final concentration of TRAIL was 1000 ng/mL in all cases. After 24 h, cell death was quantified by annexin-V staining. Graphs show the mean ± SD of at least three independent experiments. * p < 0.05, ** p < 0.01, *** p < 0.001.