Metastasis-associated protein 2 (MTA2) knockdown did not influence viability or proliferation of renal cell carcinoma (RCC) cells. (A) MTA2 knockdown expression in shLuc or shMTA2 of 786-O, ACHN, and Caki-1 cells was verified using western blotting. (B) Cell viability of shLuc or shMTA2-786-O, ACHN, and Caki-1 cells was evaluated using an MTT assay after 24 and 48 h. (C) Flow cytometry analysis of shLuc–or shMTA2–786-O, ACHN, and Caki-1 cells. β-Actin was used as an internal control for protein equal loading. Values are expressed as the mean ± SE of three independent experiments. ** p < 0.01 compared with shLuc cells.